TABLE 1.
Log of spore killing under conditions specified for sterilization and disinfectiona
| Conditions and treatment | Timeb | Mean log killing ± SE (no. of expts)
|
|||
|---|---|---|---|---|---|
| Tubing | Screws | Bursc | Avgd | ||
| Sterilization | |||||
| Water | 10 h | 0.25 ± 0.09 (8) | 0.50 ± 0.09 (8) | 0.38 ± 0.06 (16) | |
| Cupric ascorbate | 10 h | 2.62 ± 0.20 (6) | 3.24 ± 0.17 (8)e | 2.93 ± 0.14 (14) | |
| Exspor | 10 h | 3.47 ± 0.30 (4) | 3.95 ± 0.30 (4) | 3.71 ± 0.16 (8) | |
| Cidexplus | 10 h | 3.28 ± 0.25 (4) | 3.50 ± 0.25 (4) | 3.39 ± 0.27 (8) | |
| Renalin | 11 h | 4.34 ± 0.37 (4) | 5.16 ± 0.30 (6) | 4.75 ± 0.24 (10) | |
| Wavicide-01 | 10 h | 3.81 ± 0.34 (4) | 3.44 ± 0.34 (4) | 3.62 ± 0.24 (8)f | |
| Disinfection | |||||
| Water | 30 min | 0 (5) | 0 (6) | 0 (5) | |
| Cupric ascorbate | 30 min | 2.36 ± 0.22 (5) | 3.29 ± 0.20 (6)e | 1.51 (5) | 2.83 ± 0.13 (11) |
| Expor | 3 min | 3.36 ± 0.27 (5) | 4.15 ± 0.30 (6)e | 0.49 (5) | 3.76 ± 0.20 (11) |
| Cidexplus | 20 min | 3.07 ± 0.22 (5) | 4.00 ± 0.21 (6)e | 2.43 (5) | 3.54 ± 0.06 (11) |
| Cavicide | 10 min | 0.73 ± 0.29 (5) | 0.80 ± 0.27 (6) | −0.22 (5) | 0.76 ± 0.20 (11) |
| Clorox | 5 min | 1.95 ± 0.39 (5) | 1.66 ± 0.35 (6) | −0.40 (5) | 1.81 ± 0.26 (11) |
| Lysol | 10 min | 0.62 ± 0.18 (5) | 0.18 ± 0.17 (6) | −0.33 (5) | 0.40 ± 0.12 (11) |
| Wavicide-01 | 10 min | 1.63 ± 0.40 (3) | 0.63 ± 0.28 (6) | −0.11 (3) | 1.13 ± 0.24 (9)f |
Spore survival was calculated by adding the numbers of spores recovered in fractions a, b, and c of the test described in the text. Log of spore killing was calculated as the log of the number of spores surviving treatment with water for 30 min minus the log of the number of spores surviving after each treatment.
Incubation was done for the times specified by the manufacturer’s label instructions, generally consisting of a short time (on the order of minutes) for disinfection and an extended time (10 to 11 h) for sterilization.
Metal flaking and corrosion products made spore killing on burs imprecise, and the data were omitted from further analysis.
Average of spore killing by each disinfectant in tubing and screws.
Significant difference (P > 0.95) between tubing and screws for the same treatment.
Significant difference (P > 0.05) between conditions for sterilization and disinfection for the same disinfectant.