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. 2023 Jan 24;8(2):e164608. doi: 10.1172/jci.insight.164608

Figure 7. scAAV9-Abt1 delivery in Ighmbp2nmd/nmd mice does not modify Ighmbp2 splicing nor IGHMBP2 protein levels.

Figure 7

WT (+/+), Ighmbp2+/nmd, Ighmbp2nmd/nmd, and Ighmbp2nmd/nmd mice injected with scAAV9-Abt1 (4 × 1011 viral genomes) are evaluated. (A) Representative RT-PCR of P10 spinal cords. M, New England Biolabs 100 bp DNA marker. (B) Representative Western blot of P10 spinal cords. HEK293T cells transfected with ssAAV9-IGHMBP2 served as the IGHMBP2 positive control. Actin served as the loading control. In total, 20 μg of protein was added to each lane. (C) Quantification of IGHMBP2/actin ratio based on Western blots of P10 spinal cords. Densitometry data determined by Western blot were analyzed by PROC GLM of Statistical Analysis Systems (v9.4). F test was used to determine treatment effects, and Duncan multiple range test was used for differences between groups; data are shown as mean ± SEM. n = 6.