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. 2023 Jan 24;8(2):e162060. doi: 10.1172/jci.insight.162060

Figure 6. Overexpression of IGF2BP3 in vivo promotes kidney fibrosis and β-catenin activation after UUO.

Figure 6

(A) Experimental design. The green arrow indicates the timing of injecting AAV-Ctrl or AAV-BP3. The black arrow indicates the timing of UUO surgery. (B and C) Representative Western blot (B) and quantitative data (C) show the expression of Flag and IGF2BP3 in different groups as indicated. ***P < 0.001 versus the sham injected with AAV-Ctrl group; †††P < 0.001 versus the UUO injected with AAV-Ctrl group (n = 5, ANOVA with Student-Newman-Keuls test). (D) Micrographs show Flag and IGF2BP3 expression in UUO kidney. Arrows indicate positive staining. Scale bar, 50 μm. (E–H) Representative Western blot (E) and quantitative data show the expression of vimentin (F), fibronectin (G), and α-SMA (H) proteins in different groups as indicated. *P < 0.05, **P < 0.01, ***P < 0.001 versus the sham-injected with AAV-Ctrl group; †P < 0.05, ††P < 0.01 versus the UUO injected with AAV-Ctrl group (n = 5, ANOVA with Student-Newman-Keuls test). (I) Micrographs show the expression of vimentin, fibronectin, α-SMA, and collagens at 7 days after UUO in different groups. Arrows indicate positive staining. Scale bar, 50 μm. (J) Graphical presentation of renal fibrotic lesions in different groups. ***P < 0.001 versus the sham injected with AAV-Ctrl group; †††P < 0.001 versus the UUO injected with AAV-Ctrl group (n = 5, ANOVA with Student-Newman-Keuls test). (K and L) Representative Western blots (K) and quantitative data (L) show the expression of β-catenin, active β-catenin, MMP-7, Snail1, p-H3, p53, and p16INK4A in different groups as indicated. *P < 0.05, **P < 0.01, ***P < 0.001 versus the sham injected with AAV-Ctrl group; †P < 0.05, ††P < 0.01, †††P < 0.001 versus the UUO injected with AAV-Ctrl group (n = 5, ANOVA with Student-Newman-Keuls test).