Skip to main content
. 2023 Feb 8;8(3):e165369. doi: 10.1172/jci.insight.165369

Figure 5. Notch gain or loss of function reduces hepatocyte MCP-1 levels.

Figure 5

(A) Ccl2 gene expression in WT primary hepatocytes transduced with adenovirus encoding GFP or NICD (n = 6 biologic replicates/group). (B) MCP-1 protein in WT primary hepatocytes transduced with adenovirus encoding GFP or NICD (n = 3 biologic replicates/group). MCP-1 and actin blots are derived from samples run on the same gel, with filter paper cut and probed separately. (C) Circulating MCP-1 in WT primary hepatocytes transduced with adenovirus encoding GFP or NICD (n = 6 biologic replicates/group). (D) Liver Ccl2 gene expression in Cre- and L-NICD male mice (n = 8 mice/group). (E) MCP-1 protein levels in Cre- and L-NICD male mice (n = 6 mice/group). MCP-1 and actin blots are derived from samples run on the same gel, with filter paper cut and probed separately. (F) Ccl2 gene expression in livers from NASH diet–fed Cre- and hepatocyte-specific Notch loss-of-function (L-DNMAM) male mice (n = 6 mice/group). (G) MCP-1 protein levels in livers from NASH diet-fed Cre- and hepatocyte-specific Notch loss-of-function (L-DNMAM) male mice (n = 4 mice/group). MCP-1 and actin blots are derived from the same samples run contemporaneously in parallel gels. All data are shown with group means ± SEM; *, P < 0.05, **, P < 0.01, ***, P < 0.001, ****, P < 0.0001 by 2-tailed t test.