Figure 3. Combination therapy of MK-4482 and PF-07321332 significantly reduces viral replication in the lower respiratory tract of SARS-CoV-2–infected rhesus macaques.

Viral RNA load in BAL (A) and total lung lobes (C). BAL samples (n = 5) were collected on 1, 2, and 4 dpi, and lung samples (n = 30) were collected following necropsy on 4 dpi. Viral RNA loads from BAL and lung samples were determined by quantitative RT-PCR targeting sgE RNA. Infectious titers in BAL (B) and total lung lobes (D). BAL samples (n = 5) were collected on 1, 2, and 4 dpi, and lung samples were collected following necropsy on 4 dpi. Infectivity was determined using a TCID₅₀ assay and is presented as TCID₅₀/mL. Samples from each lung lobe (n = 6) of each animal were assessed individually and compiled for analysis (total n = 30) (C and D). Data represent mean. Statistical differences in viral load and infectious virus titers in the BAL were assessed by 2-way ANOVA using Tukey’s multiple comparisons test. Statistical differences in total lung lobes were analyzed with Kruskal-Wallis with Dunn’s multiple comparisons test (P values, * = 0.01 to 0.05, *** = 0.0001 to 0.001, **** = <0.0001). Nonsignificant results are not indicated.