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. 2023 Feb 22;8(4):e161003. doi: 10.1172/jci.insight.161003

Figure 5. Oligodendrocyte loss, demyelination, and axonal loss after AQP4 immunization.

Figure 5

(A–C) Immunostaining for Olig2 (A), MBP (B), and NF-H (C) in the spinal cord of naive, pEmpty(C/P+), pAQP4(C/P–), and pAQP4(C/P+) mice. (D) Merged images of MBP and NF-H coimmunostaining. (E and F) Quantification of Olig2 and MBP immunofluorescence intensities. (G) Quantification of the number of NF-H spots. Images are representative photomicrographs showing the ventrolateral white matter of cervical spinal cord cross sections from 5 mice per group. Nuclei were counterstained with DAPI. Data are mean ± SEM; n = 5 per group. **P < 0.01, ***P < 0.001, 1-way ANOVA with post hoc Tukey’s test. Scale bar: 50 μm.