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. 2023 Feb 22;8(4):e166655. doi: 10.1172/jci.insight.166655

Figure 2. FMT from butyrate-treated lean donor mice attenuates HFD-induced weight gain and improves insulin resistance in recipient mice.

Figure 2

(A) Mice were fed an HFD without or with 5% (w/w) sodium butyrate prevention for 6 weeks. After this, fresh feces were collected weekly and used for FMT to gut microbiota-depleted recipient mice that were fed an HFD for 6 weeks. (B) BW was measured weekly and the BW change was calculated (n = 8). (C) At the end of the experiment, body composition was measured by MRI (n = 8). (D) The average food intake per day throughout the intervention period was calculated (n = 8). (E) Fasting glucose (n = 7–8) and (F) insulin (n = 8) plasma levels were measured. (G) They were then used for calculation of HOMA-IR (n = 7–8). Data are shown as means ± SEM; statistical significance between 2 groups was determined with 2-tailed Student’s unpaired t test; For data represented in the line graphs showing the changes over time for a continuous variable, statistical significance between 2 groups at each time point was determined using 2-tailed Student’s unpaired t test. *P < 0.05, **P < 0.01; Butyrate vs. Control.