Figure 8. Higher BMI is associated with increased vacuolation and decreased nuclear TFEB in PTECs of patients with CKD.
(A) Representative images of PAS staining and immunohistochemical staining for TFEB, LAMP1, and SQSTM1/p62 on kidney specimens obtained from obese and nonobese patients. Specimens were counterstained with hematoxylin. Magnified images are shown in the insets (original magnification, ×400). Bars: 50 μm. (B and C) Correlation between BMI and the severity of vacuolar formation (B) (n = 146) and between the percentage of PTECs exhibiting TFEB nuclear translocation and either BMI or the number of vacuoles (C) (n = 30). Relationships were examined using Pearson’s correlation and the corresponding P values. SQSTM1, sequestosome 1.