TABLE 2.
The information of antibody
| Name | Article number | Source | Dilution rate | Molecular weight | Transfer film time | Company |
|---|---|---|---|---|---|---|
| TNF‐α | 60,291‐1‐Ig | Mouse | 1:5000 | 17–22 KDa | 40 min | Proteintech (Chicago, IL, USA) |
| IL‐1β | ab254360 | Rabbit | 1:1000 | 30 KDa | 50 min | Abcam (Cambridge, UK) |
| IL‐6 | 21,865‐1‐AP | Rabbit | 1:500 | 20 KDa | 40 min | Proteintech (Chicago, IL, USA) |
| MMP‐3 | 17,873‐1‐AP | Rabbit | 1:1000 | 45–47 KDa | 70 min | Proteintech (Chicago, IL, USA) |
| MMP‐13 | 18,165‐1‐AP | Rabbit | 1:1000 | 65–70 KDa | 90 min | Proteintech (Chicago, IL, USA) |
| CollagenII | ab188570 | Rabbit | 1:5000 | 141 KDa | 150 min | Abcam (Cambridge, UK) |
| aggrecan | ab36861 | Rabbit | 1 μg/mL | 110 KDa | 130 min | Abcam (Cambridge, UK) |
| NLRP3 | ab263899 | Rabbit | 1:1000 | 118 KDa | 150 min | Abcam (Cambridge, UK) |
| Caspase‐1 | ab179515 | Rabbit | 1:1000 | 45/10/12 KDa | 75 min | Abcam (Cambridge, UK) |
| β‐Actin | 66,009‐1‐Ig | Mouse | 1:5000 | 42 KDa | 60 min | Proteintech (Chicago, IL, USA) |
| HRP goat anti‐Rabbit IgG | SA00001‐2 | Rabbit | 1:6000 | / | 90 min | Proteintech (Chicago, IL, USA) |
| HRP goat anti‐mouse IgG | SA00001‐1 | Mouse | 1:5000 | / | 90 min | Proteintech (Chicago, IL, USA) |