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. Author manuscript; available in PMC: 2024 Feb 1.
Published in final edited form as: Chembiochem. 2022 Nov 4;24(3):e202200487. doi: 10.1002/cbic.202200487

Figure 3. Generating a site-specific semi-synthetic EGFR in live cells.

Figure 3.

A) Coomassie and in gel fluorescence of AvaN −/+ the synthetic peptide. B) Comparison of splicing with recombinant vs synthetically modified AvaN. HEK293T cells were transfected with Flag-NpuC-EGFR and then treated with 20 μM of HA-AvaN or TAMRA-HA-AvaN for 60 minutes. The membrane fraction was then extracted, separated on SDS-PAGE and analyzed by western blot (anti-Flag – green, anti-HA – red, anti-Na/K ATPase – gray scale). C) Live cell images of HEK293T cells transfected with Flag-NpuC-EGFR and treated with TAMRA-HA-AvaN (red) and Hoechst (blue).