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. 2023 Jan 26;24(3):439–451. doi: 10.1038/s41590-022-01418-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — (a) A representative immunoblot showing NGF levels in the hind paw skin of Snx25^fl/fl mice and Snx25^Cx3cr1-cKO mice after two-week TAM treatment (schedule was shown in upper panel). Semi-quantitative analyses of the NGF levels are shown (n = 5, p = 0.059). (b) Flow cytometry strategy to sort dMacs, dMonos and dDCs from the back skin of mice using CD11b, CD24, Ly6C, CD64, MHCII, and lineage (CD3, CD19, NK1.1, TER119, Ly6G) marker expression. (c) Proportion of myeloid population (dMacs, dMonos, and dDCs) (% each cell / CD45⁺ CD11b⁺ Lin^- × 100) in the back skin of WT or Snx25^+/− mice (WT: n = 4; Snx25^+/−: n = 6). dMacs, p = 0.577; dMonos, p = 0.289; dDCs, p = 0.898. (d) Expression patterns of Snx25 and Ngf in dMonos of WT and Snx25^+/− mice (WT: n = 3; Snx25^+/−: n = 3). Snx25, p = 0.009; Ngf, p = 0.328. (e) Expression patterns of Snx25 and Ngf in dMonos of Snx25^fl/fl and Snx25^Cx3cr1-cKO mice (Snx25^fl/fl: n = 3; Snx25^Cx3cr1-cKO: n = 3). Snx25, p = 0.748; Ngf, p = 0.988. (f) Expression patterns of Snx25 and Ngf in dDCs of WT and Snx25^+/− mice (WT: n = 4; Snx25^+/−: n = 4). Snx25, p = 0.001; Ngf, p = 0.256. (g) Expression patterns of Snx25 and Ngf in dDCs of Snx25^fl/fl and Snx25^Cx3cr1-cKO mice. (Snx25^fl/fl: n = 3; Snx25^Cx3cr1-cKO: n = 3). Snx25, p = 0.062; Ngf, p = 0.804. Results are represented as mean ± SEM. Statistical analyses were performed using the two-tailed Student’s t-test (c), Welch’s t-test (a, d, e, f, and g). *p < 0.05, **p < 0.01. n.s., not significant.

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