Extended Data Fig. 4. Tumour-infiltrating immune cells of KPC tumours from gnotobiotic mice.
a, Flow cytometric gating strategy to classify immune subsets (left panel) or intracellular cytokines and coinhibitory receptors (right panel). Immune subsets were determined using the following marker combinations on live CD45+ immune cells: CD8+ T cells (CD11b−CD3+CD8+); CD4+ T cells (CD11b−CD3+CD4+); neutrophils (CD11b+Ly6G+); and macrophages (CD11b+Ly6G−F4/80+). b, Immune subsets or cytokine profiles and PD-1 expression of immune cells from orthotopic tumours obtained from gnotobiotic mice colonized with R or NR microbiota was analysed at day 20 of the experiment. Each symbol represents one mouse. Three to five independent experiments were pooled (n = 11 to 23 in total). Error bars indicate SEM, significant p-values are indicated and were determined by two-tailed t-test.