Extended Data Fig. 5. CD4+ and CD8+ T cells are dispensable for the efficacy of 3-IAA and FIRINOX.
R-microbiota-colonized gnotobiotic mice were injected with KPC tumour cells orthotopically. Before treatment with FIRINOX, mice were injected with either isotype control antibody or CD8-depleting antibody every third day, as indicated. The tumour weight (a) and the depletion efficacy of intratumoral CD8+ T cells (b) as relative to total immune cells (CD45+), determined by flow cytometry, is shown at day 20 of the experiment (n = 3). c, As in a, except that CD4+ and CD8+ T cells were depleted simultaneously (n = 3 or 4). d, R-microbiota-colonized gnotobiotic mice with orthotopic KPC tumours were treated with FIRINOX, FIRINOX + tryptophan-high diet (d8–12) + isotype control antibody or FIRINOX + tryptophan-high diet (d8–12) + CD4/CD8-depleting antibody (n = 4 or 5). Tumour weight at day 20 of the experiment is depicted. e, As in c, except that SPF mice were treated with 3-IAA +/− FIRINOX and CD4/CD8-depleting or isotype control antibody (n = 4). Tumour weight is depicted at day 20 of the experiment. Each symbol represents one mouse. One experiment each was performed. Error bars indicate SEM, significant p-values are indicated and were determined by two-tailed t-test (a,b), one-way ANOVA followed by Dunnett’s (c,d) post-hoc test or Kruskal–Wallis test followed by Dunn’s post-hoc test (e).