Figure 2.

QPRK mutation causes sensitivity to cell wall stress in S. cerevisiae. (A–E) Cultures of the indicated S. cerevisiae strains were serially diluted and spotted on YPAD agar plates containing indicated concentrations of (A) methyl methanesulfonate (MMS), (B) calcofluor white (CFW), (C) micafungin (MF), (D) caspofungin (CF), and (E) MF or MF + sorbitol. “#1” and “#2” refer to independent isolates of cdc14^hm. yen1Δ mus81Δ is a positive control for MMS sensitivity (Ho et al., 2010). All plates were incubated at 30°C for 72 h. Images are representative of three biological replicates. “Wild-type” is W303. (F) Immunoblot detecting phosphorylated, active form of Slt2 (pSlt2) from cell lysates of mid-log phase cells with anti-p44/42 MAP kinase antibody. pSlt2 signals were corrected based on the G6PDH (glucose-6-phosphate dehydrogenase) load control and then normalized to the untreated wild-type sample to generate the Relative pSlt2 value, which is an average of two independent trials.