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. 2023 Mar 1;13:3431. doi: 10.1038/s41598-023-30587-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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¹H NMR spectra of: free palindromic duplex d(CGTAGCTACG)₂, codenamed U2 (A); U2 duplex interacting with C-2045 (B). In all cases, the stoichiometry of the examined complexes was dsDNA/ligand 1:1.5 mol/mol. Red asterisks mark the imino protons of the remaining, free dsDNA duplexes. The U2 was not analyzed by means of 2D NMR spectroscopy, hence the imino protons were not labelled.