Table 2.
Observed d(CGATATCG)2:C-1311 (D2L) and d(CCCTAGGG)2:C-1311 (D3L) intermolecular NOE contacts. The intensities were classified as weak/medium/strong on the basis of the integration of the respective crosspeaks in the 2D NOESY spectra (τm = 150 ms) of the complexes and due to the lack of an internal standard of the DNA/ligand relaxation.
| No. | C-1311 proton | DNA proton | D2L (intensity) | D3L (intensity) |
|---|---|---|---|---|
| NOE contacts involving aromatic protons of the ligand | ||||
| 1 | L-H1 | B-T4CH3 | – | + (medium) |
| 2 | L-H1 | A-A5H8 | – | + (medium) |
| 3 | L-H3 | B-T4CH3 | + (weak) | – |
| 4 | L-H3 | B-T4H1′ | + (medium) | + (weak) |
| 5 | L-H3 | B-T4H2′ | + (weak) | – |
| 6 | L-H3 | B-T4H2″ | + (strong) | + (medium) |
| 7 | L-H3 | B-T4H6 | + (medium) | + (weak) |
| 8 | L-H3 | B-A5H8 | + (strong) | + (medium) |
| 9 | L-H4 | B-T4H2″ | + (strong) | + (medium) |
| 10 | L-H4 | B-T4H6 | + (medium) | + (medium) |
| 11 | L-H4 | B-A5H1′ | + (medium) | – |
| 12 | L-H4 | B-A5H2′ | – | + (strong) |
| 13 | L-H4 | B-A5H8 | + (medium) | + (medium) |
| 14 | L-H7 | A-T4CH3 | + (medium) | + (medium) |
| 15 | L-H7 | A-T4H1′ | + (medium) | + (weak) |
| 16 | L-H7 | A-T4H2″ | + (medium) | + (medium) |
| 17 | L-H7 | A-T4H6 | + (medium) | + (medium) |
| 18 | L-H7 | A-A5H8 | + (medium) | + (weak) |
| 19 | L-H9 | A-T4CH3 | + (strong) | + (medium) |
| 20 | L-H9 | A-A5H8 | + (strong) | + (medium) |
| 21 | L-H10 | A-T4CH3 | + (medium) | + (medium) |
| 22 | L-H10 | A-T4H1′ | – | + (medium) |
| 23 | L-H10 | A-A5H8 | + (strong) | – |