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. 2023 Mar 1;14:1161. doi: 10.1038/s41467-023-36747-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a RNA level of Ccl28 in IRI-Alkbh5^fl/flKsp^Cre group and IRI-Alkbh5^fl/fl group was examined by RT-qPCR. n = 3 biologically independent animals. b Protein level of CCL28 in IRI-Alkbh5^fl/flKsp^Cre group and IRI-Alkbh5^fl/fl group was examined by western blot. c Representative IHC staining data of CCL28 in kidney biopsies from IRI mice. (Magnification: ×400, Scale bar: 40 μm). d Serum CCL28 level was examined by ELISA. n = 5 biologically independent animals. e m6A modification of Ccl28 mRNA was detected by MeRIP-qPCR analysis using anti-IgG and anti-m6A antibodies. n = 3 biologically independent animals. f Relative enrichment of Ccl28 mRNA associated with ALKBH5 protein was identified by RIP assays using anti-IgG and anti-FLAG antibodies. n = 4 biologically independent experiments. g Relative activity of the pGL3-empty, pGL3-CDS, pGL3-5’UTR, and pGL3-3’UTR luciferase reporter in Ad-Control and Ad-ALKBH5 groups. n = 3 biologically independent experiments. h Relative activity of the pGL3-empty, pGL3-WT, pGLMut1, pGLMut2, pGLMut3, and pGLMut4 luciferase reporter in Ad-Control and Ad-ALKBH5 groups. n = 3 biologically independent experiments. i ALKBH5-silenced and control cells were treated with actinomycin D and harvested at 0, 2, 4, and 6 h. RNA decay rate was determined to estimate the stability of Ccl28 mRNA (normalized to the expression at 0 h). n = 3 biologically independent experiments. j RT-qPCR analysis of Ccl28 in mRTECs after different insulin-like growth factor 2 binding proteins (IGF2BPs) knockdown compared to NC treatment. n = 3 biologically independent experiments. k Western blot analysis of CCL28 after IGF2BP2 knockdown in H/R-treated mRTECs. Three biological repeated immunoblots have been performed. l RT-qPCR analysis of RIP assays in H/R-treated mRTECs showing the direct binding between the IGF2BP2 protein and Ccl28 mRNA. n = 3 biologically independent experiments. m ALKBH5-silenced with/without IGF2BP2-silenced cells were treated with actinomycin D and harvested at 0, 2, 4, and 6 h. RNA decay rate was determined to estimate the stability of Ccl28 mRNA (normalized to the expression at 0 h). n = 3 biologically independent experiments. Data are shown as means ± SD. Unpaired two-tailed Student’s t-test for (a), (d–h), (j), (l).