Fig. 3.

Increasing spread of Pkm2 expression in macrophages and keratinocytes during healing of cutaneous wound. Serial sections from 5-day wounds isolated from C57BL/6 J mice using antibodies directed against Pkm2 (brown signals, field yellow arrows) in conjugation with antibodies directed against: Ly6B.2 (activated PMN/monocytes; red signals, red arrows) (A), or in conjugation with antibodies directed against F4/80 (macrophage; red signals, red arrow) (B). A full wound section is shown at the left side (scale bars: 1000 µm) and the marked region is shown in the middle part (scale bars: 100 µm). A detailed image at higher magnification is shown at the right side (scale bars: 50 µm). (C) Evaluation of Pkm2 expression in neutrophils and mononuclear phagocytes in wound sections obtained from three different mice (n = 3) on days 3 and 5 post injury. Ly6B.2 or F4/80 cells were assessed for expressing Pkm2 in 0.72 mm². At least four images taken from wound edge (WE) and two from wound bed (WB) were evaluated for each section. ***P < 0.001; (one-way ANOVA) (Bonferroni’s post-hoc test). Ly6B.2 positive cells are rarely detectable in the wound edge on day 5 post injury and hence not assessed (n/a). Staining of Pkm2 alone is shown in (D). A full wound section is shown at the left side (scale bars: 1000 µm) and a detailed image of the marked region is shown at the right side (scale bars: 100 µm). GT, granulation tissue; SC, scab; HE, hyperproliferative epithelia; WE, wound edge; WB, wound bed. The HE is marked by a yellow dash line