Table 2.

Antibodies used for immunohistochemistry

Antigen	Epitope retrieval procedure	Primary antibody	Biotinylated secondary antibody/solution
F4/80	HIER, 10 mM citrate (pH 6.0)	1:200	Rabbit anti-rat 1:200/DCSb
HIF-1α Cayman 10006421	HIER, Dako 10 mM citrate (pH 6.0)	1:10,000	Goat anti-rabbit 1:1000 Vector BA-100/TBST
Ly-6B.2	HIER, 10 mM citrate (pH 6.0)	1:400	Rabbit anti-rat 1:200/DCS
Ly-G	HIER, 10 mM citrate (pH 6.0)	1:150	Rabbit anti-rat 1:200/DCS
PKM2	HIER, 10 mM citrate (pH 6.0)	1:300	CST 8114
VEGF	HIER, Dako TRS a S1699	1:100	Donkey anti-goat 1:200 ABC

^aTarget retrieval solution; DCS

^bantibody diluent solution (AL120R; DCS, Hamburg, Germany)