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. 2022 Dec 3;128(5):833–843. doi: 10.1038/s41416-022-02090-0

Fig. 3. The role of JAK2/STAT3 pathway in the regulation of PD-L1 expression by IL-33/ST2.

Fig. 3

a Pathway enrichment analysis of potential TFs in PD-L1 promoter region. b Western blot showed PD-L1 expression and JAK2/STAT3 phosphorylation in tumor cells after ST2 knockdown. c The protein level of JAK2/STAT3 activation and PD-L1 expression in tumor cells stimulated by IL-33 after knockdown of ST2 (25 ng/ml). d Western blot was used to analyze the activation of JAK2/STAT3 pathway and the expression of PD-L1 in tumor cells stimulated by IL-33 after overexpression of ST2. e Western blot was used to analyze the effect of AG490 on the phosphorylation of JAK2/STAT3 pathway and the expression of PD-L1 under different concentrations of IL-33 stimulation. f The effect of IL-33 stimulation on the expression of PD-L1 and p-STAT3 in HN6 cells was analyzed by cellular immunofluorescence. g, h Immunofluorescence analysis of the effect of IL-33 stimulation on the expression of PD-L1 and p-STAT3 in 3D tumor spheres of HN6. Results are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. p = two-tailed t test.