Fig. 2. ZBP1/p-MLKL mediated necroptosis existed in macrophages in BA livers.

A The correlation between ZBP1 and p-MLKL in human liver tissues. B Hepatic ZBP1 mRNA level detected by RNA sequencing and qRT-PCR were compared between biliary atresia (BA, RNA-seq: n = 31, qRT-PCR: n = 12) and normal adjacent non-tumor livers (HC, RNA-seq: n = 20, qRT-PCR: n = 7). C Representative images of p-MLKL (green), CD86 (pink) and CD11b (red) staining. The nuclei were stained with DAPI (blue). Yellow arrows: p-MLKL⁺CD86⁺CD11b⁺ cells. Scale bar: 30 μm. D Representative images of ZBP1 (green), CD68 (pink) and CD11b (red). The nuclei were stained with DAPI (blue). Yellow arrows: ZBP1⁺CD68⁺CD11b⁺ cells. Scale bar: 30 μm. E, F Quantitative results of p-MLKL⁺(E) and ZBP1⁺ (F) macrophage number in BA livers. G Representative images of p-MLKL (green), ZBP1 (pink) and CD11b (red) staining in BA livers. The nuclei were stained with DAPI (blue). Yellow arrows: p-MLKL⁺ZBP1⁺CD11b⁺ cells. Data are presented as the mean ± SEM. **p < 0.01 (versus control).