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. 2023 Mar 1;14(3):175. doi: 10.1038/s41419-023-05615-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The schedule of mouse model. B Representative images of p-MLKL (pink) and F4/80 (red) staining in BDL livers. EGFP (green) indicated bone marrow-derived cells. The nuclei were stained with DAPI (blue). Yellow arrows: p-MLKL⁺F4/80⁺EGFP⁺ cells. Scale bar: 15 μm. C Representative images of ZBP1 (pink) and F4/80 (red) staining in BDL livers. EGFP (green) indicated bone marrow-derived cells. The nuclei were stained with DAPI (blue). Yellow arrows: ZBP1⁺F4/80⁺EGFP⁺ cells. Scale bar: 15 μm. D, E Quantitative results of p-MLKL⁺ (D) and ZBP1⁺ (E) macrophage number in BDL livers. Data are presented as the mean ± SEM.