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. 2023 Mar 1;14(3):175. doi: 10.1038/s41419-023-05615-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A, B ZBP1 mRNA (A) and protein (B) levels were measured by qRT-PCR and western blot in BMDMs treated with 100 μmol/L sodium taurocholate hydrate (TCA), glycocholic acid hydrate (GCA), and sodium glycodeoxycholate (GDCA) for 6 h, respectively. C Zbp1 mRNA level was measured by qRT-PCR in mouse BMDMs treated with the indicated concentrations of GDCA for 6 h. D Zbp1 mRNA level was measured by qRT-PCR in mouse BMDMs treated with 100 μmol/L GDCA for different time points. E, F ZBP1 protein level was measured in mouse BMDMs treated with the indicated concentrations of GDCA (E) or different time points (F). All results were confirmed by at least three independent experiments. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (versus control).