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. 2023 Mar 1;14(3):175. doi: 10.1038/s41419-023-05615-4

Fig. 7. GDCA increased ZBP1 expression in mouse BMDMs through S1PR2.

Fig. 7

A Hepatic S1PR2 mRNA level detected by RNA sequencing was compared between biliary atresia (BA, n = 31) and normal adjacent non-tumor livers (HC, n = 20). B mRNA level of S1pr2 in mouse livers was measured by qRT-PCR. C Violin plots showed S1pr2 expression of each cell clusters in scRNA-seq data. D S1pr2 mRNA level was measured by qRT-PCR in mouse BMDMs treated with 100 μmol/L sodium glycodeoxycholate (GDCA) for 6 h. E Mouse BMDMs were pretreated with S1PR2 antagonist JTE-013 (10 μmol/L) for 1 h, followed by 100 μmol/L GDCA for 6 h, and Zbp1 mRNA level was measured by qRT-PCR. F Effect of S1pr2 siRNA on Zbp1 mRNA expression in response to 100 μmol/L GDCA for 6 h. G ZBP1 protein level was measured in mouse BMDMs pretreated with JTE-013, followed by GDCA. H Effect of S1pr2 siRNA on ZBP1 protein expression in response to 100 μmol/L GDCA for 6 h. All results were confirmed by at least three independent experiments. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 (versus control). #p < 0.05, ###p < 0.001 (versus GDCA treated alone or GDCA with siNC).