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. 2023 Mar 1;14:1167. doi: 10.1038/s41467-023-36818-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Representative whole-cell patch-clamp recordings of currents elicited by mechanical stimulation (−60 mV) in human iPSC-derived sensory neurons transfected with scrambled, UBE3A, or PIEZO2 siRNAs. b Current densities elicited by maximum displacement of siRNA-transfected iPSC-derived neurons. Bars are mean ± SD. Kruskal-Wallis (H = 13.9; p = 9.6⁻⁴) and Dunn’s multiple comparisons test. c Representative whole-cell patch-clamp recordings of currents elicited by mechanical stimulation (−60 mV) of control and LA-treated iPSC-derived sensory neurons (two-day LA supplementation; 50 µM for 24 h and 100 µM for another 24 h). d Current densities elicited by maximum displacement of control and LA-treated iPSC-derived neurons. Bars are mean ± SD. Two-tailed unpaired t-test (t = 4.57). e Boxplots show the displacement thresholds required to elicit mechanocurrents in of control and LA-treated iPSC-derived neurons. Two-tailed Mann-Whitney test (U = 9). f Top, current-clamp recordings of membrane potential changes elicited by indentation of control and LA-treated iPSC-derived neurons. Bottom, membrane potential peak vs. mechanical indentation of independent iPSC-derived neurons. At the top, boxplots show the displacement threshold required to elicit an action potential in these neurons. Two-tailed Mann-Whitney test (U = 0.5). g Representative whole-cell patch-clamp recordings elicited by mechanical stimulation (−60 mV) of rapidly, intermediate, and slowly inactivating currents of neurotypical and AS DPSC-derived neurons, with or without LA supplementation (two-day LA supplementation; 50 µM for 24 h and 100 µM for another 24 h). h Current densities elicited by maximum displacement from neurotypical and AS DPSC-derived neurons, ± LA supplementation, classified by their time constant of inactivation. Bars are mean ± SD. Two-way ANOVA (F = 45.23; p = 5.69⁻¹³) and Tukey multiple-comparisons test. Neurotypical (three individuals), AS (five individuals), and AS + LA (three individuals). Boxplots show mean (square), median (bisecting line), bounds of box (75^th to 25^th percentiles), outlier range with 1.5 coefficient (whiskers), and minimum and maximum data points. n is denoted in each panel. Post-hoc p-values are denoted in the corresponding panels. Source data are provided as a Source Data file.