Figure 2.

Susceptibilities of RVFV RNPs to RNAses. Huh7 cells were infected with RVFV at an MOI of 3 and mock-irradiated (UV -) or irradiated with UV light (UV +) at 8 h p.i. Cell extracts were prepared and treated with RNAse A/T1 mixture (RNAse +) for 3 h at 37˚C. After RNAse digestion, viral RNAs were extracted and subjected to Northern blot analysis using digoxigenin-labeled RNA probes, each of which specifically binds to genomic L (left panel), M (middle panel) or S RNA (right panel). Strong RNA signals indicated by arrows in the RNAse-untreated samples represent the viral genomic RNAs.