Figure 5.

Profile of viral RNA segments packaged into purified RVFV and RVFV Δ19 particles. Proportions of genomic and antigenomic RVFV RNA segments in (A) RVFV and (B) RVFV Δ19-infected cells and purified virus particles. Huh7 cells were infected with RVFV or RVFV Δ19 at an MOI of 2. Intracellular lysates and culture supernatants were collected at 8 h p.i. Virus particles released in the culture supernatant were purified by sucrose gradient ultracentrifugation. The copy numbers of genomic (G) and antigenomic (AG) L, M, S RNAs in intracellular lysate and in purified virions were determined by strand-specific RT-qPCR. The proportions of genomic and antigenomic RNAs in virus-infected cells and in purified virions are represented as a percentage of total viral RNA for each segment. Data represent the means of biological triplicates, and error bars indicate the standard deviations.