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Generation of c-Fos KO rats. (a) CRISPR/Cas design for c-Fos KO rats. Two gRNA were used for the generation of c-Fos KO rats. The red characters show the target sequence of gRNA, and the green characters show the first ATG of the c-Fos gene. (b) DNA sequence analysis of c-Fos KO rats. The genome of c-Fos KO rats was amplified with the sense primer 5′-ggcgagctgttcccgtcaatccc and the antisense primer 5′-gtccagaatcgctactcacctgctctac. The PCR product was sequenced with the antiprimer.
