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. 2023 Feb 16;16:1116000. doi: 10.3389/fnmol.2023.1116000

Figure 3.

Figure 3

The GAL4/UAS system represents the most widely used binary expression system. In the parental generation, one fly carries GAL4 under a specific promoter and the other a genetic sequence downstream of UAS. When these flies are crossed, the progeny will express GAL4 in all tissues, in which the promoter is utilized. There, it acts as a transcription factor and binds to UAS, leading to expression of the downstream sequence.