Table 1.
Differenciation cluster (CD) | MFI | inhibition (1%) | |
---|---|---|---|
MFI | inhibition (1%) | ||
TCR a/b | 1304 | 692 | 47 |
CD2 | 4236 | 3262 | 23 |
CD3 | 948 | 591 | 38 |
CD4 | 2005 | 954 | 52 |
CD8 | 1523 | 98 | 94 |
CD28 | 535 | 400 | 25 |
Human PBMCs were plated at 1.105 cells per well. The cells were incubated with different labeled monoclonal antibodies (anti-human TCRα/β-FITC, anti-human CD2-PE, anti-human CD3-PE, anti-human CD4-PE, anti-human CD8-PE, anti-human CD28-PE) alone or in combination with GH-ALG at 250 µg/mL. The residual labeling was analyzed by flow cytometry. The results are presented as the mean fluorescence intensity (MFI) and % signal inhibition by GH-ALG.