Skip to main content
. 2001 Oct;21(19):6395–6405. doi: 10.1128/MCB.21.19.6395-6405.2001

FIG. 3.

FIG. 3

(A) UPC2 and ECM22 were necessary for sterol-mediated regulation of ERG2-lacZ. ERG2-lacZ reporters were used to determine the contribution of each of the genes to the regulation of ERG2. “Full promoter” refers to a reporter having ERG2 promoter sequences from −751 to −93 controlling transcription of lacZ. “SRE mutated” has the same promoter except that 10 bp of the SRE have been mutated. “SRE only” has only the 11-bp SRE controlling lacZ. The plasmids were transformed into wild-type (wt; W303-1a), upc2Δ (JRY7179), ecm22Δ (JRY7180), and ecm22Δ upc2Δ (JRY7181) strains. β-Galactosidase assays were performed on the transformants after they were grown for 16 h either in minimal medium (uninduced) or in minimal medium containing 40 μg of lovastatin/ml (induced). The assay values represent the average of two determinations. (B) UPC2 and ECM22 were necessary for sterol-mediated regulation of ERG3-lacZ. An ERG3-lacZ reporter was integrated at the URA3 locus of wild-type (JRY7190), upc2Δ (JRY7191), ecm22Δ (JRY7192), and upc2Δ ecm22Δ (JRY7193) strains. β-Galactosidase assays were performed on the transformants following growth for 16 h in conventional medium (uninduced) or in the presence of 40 μg of lovastatin/ml (induced). The assay values represent the average of two determinations.