. 2023 Jan 20;43(1):39–45. [Article in Chinese] doi: 10.12122/j.issn.1673-4254.2023.01.05

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引物序列

Primer sequences for real-time PCR

Gene	Primer sequence
RUNX2: Runt-related transcription factor 2; Osx: Osterix.
PKA	Forward: 5'-ACAACCTGGACAGAGTGAAACTC-3' Reverse: 5'-CTTGATGGCGTACAGTTCCTCC-3'
CREB	Forward: 5'-CACAGACCACTGATGGACAGCA-3' Reverse: 5'-AGGACGCCATAACAACTCCAGG
RUNX2	Forward: 5'-CCTGAACTCTGCACCAAGTCCT-3' Reverse: 5'-TCATCTGGCTCAGATAGGAGGG-3'
Osx	Forward: 5'-AAGCGCTTTACCAGAAGCGA-3' Reverse: 5'-TGCAATAGGAGAGAGCGAGG-3'
β-actin	Forward: 5'-CATTGCTGACAGGATGCAGAAGG-3' Reverse: 5'-TGCTGGAAGGTGGACAGTGAGG-3'