Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-MYSM1 antibody | Abcam | ab-221725 |
| RPS24 Polyclonal antibody | ProteinTech | 14831-1-AP |
| RPS7 Polyclonal antibody | ProteinTech | 14491-1-AP |
| Ferroptosis Antibody Sampler Kit | Cell signaling Technology | 29650 |
| Brilliant Violet 421™ anti-human CD34 Antibody | BD Biosciences | 562577 |
| PE Mouse Anti-Human CD66b | BD biosciences | 561650 |
| PE Mouse Anti-Human CD33 | BD biosciences | 555450 |
| PE Mouse Anti-Human CD19 | BD biosciences | 555413 |
| RPL15 Polyclonal antibody | Proteintech | 16086-1-AP |
| RPL23 Polyclonal antibody | Proteintech | 10727-1-AP |
| Ferritin light chain Polyclonal antibody | Proteintech | 10727-1-AP |
| anti-CD90-PECy7 | BD biosciences | 561558 |
| APC-H7 Mouse Anti-Human CD45RA | BD biosciences | 560674 |
| Necroptosis Antibody Sampler Kit | Cell signaling Technology | 98110T |
| Apoptosis Antibody Sampler Kit | Cell signaling Technology | 9915T |
| hnRNP-E1 Polyclonal antibody | proteintech | 14523-1-AP |
| PCBP2 Polyclonal antibody | proteintech | 15070-1-AP |
| SLC40A1/FPN1 Polyclonal antibody | proteintech | 26601-1-AP |
| GCLM Polyclonal antibody | proteintech | 14241-1-AP |
| GCLC Polyclonal antibody | proteintech | 12601-1-AP |
| FITC Annexin V | Biolegend | 640906 |
| APC anti-human CD49c (integrin α3) Antibody | Biolegend | 343808 |
| ALAS1 Polyclonal antibody | proteintech | 16200-1-AP |
| HSP27 Polyclonal antibody | proteintech | 18284-1-AP |
| RELB Polyclonal antibody | proteintech | 25027-1-AP |
| Ferroportin/SLC40A1 Antibody - BSA Free | Novus Biologicals | NBP1-21502SS |
| BB700 Mouse Anti-Human CD133 | BD biosciences | 747638 |
| rat anti-human anti-EPCR-PE | Biolegend | 351904 |
| PE anti-human CD34 Antibody | Biolegend | 343506 |
| PE anti-human CD3 Antibody | Biolegend | 317308 |
| PE anti-human CD11b Antibody | Biolegend | 301306 |
| Monoclonal ANTI-FLAG® M2 antibody produced in mouse | Sigma Aldrich | F1804 |
| Chemicals, peptides, and recombinant proteins | ||
| Holo-transferrin Human | Sigma Aldrich | T0665 |
| Hygromycin B | Gibco | 10687010 |
| Erastin | Selleck | S7242 |
| FIN56 | Selleck | S8254 |
| FINO2 | Cayman Chemical | 25096 |
| Deferoxamine mesylate | Selleck | S5742 |
| Ferrostatin-1 | Selleck | S7243 |
| BODIPY™ 581/591 C11 | Invitrogen | D3861 |
| CellROX™ Green Reagent | Invitrogen | C10444 |
| MitoTEMPO (hydrate) | Cayman Chemical | 16621 |
| N-acetyl-L-Cysteine | Cayman Chemical | 20261 |
| (±)-α-Tocopherol Acetate | Cayman Chemical | 28399 |
| Polyethylenimine, Linear, MW 25000, Transfection Grade (PEI 25K™) | PolySciences | 23966-100 |
| Ambion™ RNase I, cloned, 100 U/μ | Invitrogen | AM2294 |
| GlycoBlue™ Coprecipitant (15 mg/mL) | Invitrogen | AM9515 |
| Cycloheximide | Sigma Aldrich | C7698 |
| Ultra Low Range DNA Ladder | Invitrogen | 10597012 |
| SYBR™ Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) | Invitrogen | S11494 |
| RSL3 | Selleck | S8155 |
| CC100 | StemCell Technologies | 2690 |
| BioTracker Far-red Labile Fe2+ Dye | Millipore Sigma | SCT037 |
| SM-164 1mg | Cell Signaling Technology | 56003S |
| Z-VAD-FMK | Selleck | S7023 |
| Recombinant Human TNF-α 10ug | Peprotech | 300-01A |
| Mitomycin C 10mg | Selleck | S8146 |
| Iron(II) sulfate heptahydrate | F7002-250G | Sigma Aldrich |
| Liproxstatin-1 | Cayman Chemical | 17730 |
| Staurosporine | Cayman chemical | 81590 |
| Methanol | Sigma Aldrich | 179337 |
| RPMI1640 medium | Life Technologies | 11875-119 |
| Opti-MEM | Gibco | 31985-062 |
| Ficoll-Paque Premium sterile solution | GE Healthcare | 17-5442-02 |
| 0.25% Trypsin (1X) | Gibco | 15050-057 |
| Blotting nonfat dry milk | BioRad | 1706404XTU |
| Cas9 protein, recombinant | IDT | 1081061 |
| Penicillin-Streptomycin 10,000u/ml | Life Technologies | 15140-122 |
| Recombinant Human TPO | Peprotech | 300-18-100UG |
| Formamide | Sigma Aldrich | F7503 |
| TBE Buffer (Tris-borate-EDTA) (10X) | Thermo Scientific | B52 |
| Annexin-V-Binding Buffer | Biolegend | 422201 |
| L-Glutamine 200mM for cell culture | GIBCO | 25-030-081 |
| SSC (20X), RNase-free | Invitrogen | AM9763 |
| DMEM High Glucose | Life Technologies | 11965-118 |
| Critical commercial assays | ||
| Glutathione Peroxidase Assay Kit | Cayman Chemical | 703102 |
| Protein Synthesis Assay Kit | Cayman Chemical | 601100 |
| Hemin Colorimetric Assay Kit | BioVision | K672 |
| Iron Colorimetric Assay Kit | BioVision | K390 |
| Kapa Library Quantification Kit - Complete Kit (Universal) | Roche | 7960140001 |
| mMESSAGE mMACHINE™ T7 Transcription Kit | Invitrogen | AM1344 |
| MEGAclear™ Transcription Clean-Up Kit | Invitrogen | AM1908 |
| Poly(A) Tailing Kit | Invitrogen | AM1350 |
| Deposited data | ||
| Single cell RNA seq | This paper | GSE221170 |
| Ribosome Profiling | This paper | GSE221170 |
| Experimental models: Cell lines | ||
| CD34+ HSPCs | Fred Hutch | |
| HEK293T | ATCC | CRL-3216 |
| Experimental models: Organisms/strains | ||
| NBSGW | JAX Laboratory | 026622 |
| Oligonucleotides | ||
| Alt-R® Cas9 Electroporation Enhancer | IDT | 1075916 |
| Universal miRNA Cloning Linker | New England Biolabs | S1315S |
| qPCR primers and genotyping/editing efficiency testing primers | Genewiz | Table S8 |
| Chemically synthesized sgRNA | Synthego | Table S9 |
| Recombinant DNA | ||
| MGC Fully Sequenced Human SLC40A1 cDNA | Horizon Discovery | MHS6278-202801506 |
| MGC Fully Sequenced Human FTH1 cDNA | Horizon Discovery | MHS6278-202755914 |
| MGC Fully Sequenced Human ALAS1 cDNA | Horizon Discovery | MHS6278-202831218 |
| MGC Human HSPB1 Sequence-Verified cDNA | Horizon Discovery | MHS6278-202756315 |
| MGC Human RELB Sequence-Verified cDNA | Horizon Discovery | MHS6278-202801567 |
| Software and algorithms | ||
| Graphpad Prism v.9.0.1 | Graphpad | RRID:SCR_002798 |
| Biorender | Biorender | RRID:SCR_018361 |
| Flowjo | Flowjo | RRID:SCR_008520 |
| ImageJ v1.52a | Wayne Rasband | RRID:SCR_003070 |
| R | The R Foundation | |
| STAR v2.7.10a | ||
| Bowtie2 v2.3.3 | ||
| RiboCode v1.2.15 | ||
| Cutadapt v4.1 | ||
| CellRanger v7.0.0 | 10xGenomics | |
| Seurat v4.0.2 | ||
| Other | ||
| In-Fusion® HD Cloning Plus | Takara/Clontech | 638909 |
| Q5 site directed mutagenesis kit | New England Biolabs | E0554S |
| iScript™ cDNA Synthesis Kit | BioRad | 1708891 |
| Monarch® DNA Gel Extraction Kit | New England Biolabs | T1020L |
| iQ™ SYBR® Green Supermix | BioRad | 1708882 |
| P3 Primary Cell 4D Nucelofector X Kit S | Lonza | V4XP-3032 |
| SuperScript™ III Reverse Transcriptase | Invitrogen | 18080044 |
| Chromium Next GEM Single Cell 3ʹ Kit v3.1, 16 rxns | 10XGenomics | PN-1000268 |
| Chromium Next GEM Chip G Single Cell Kit, 48 rxns | 10XGenomics | PN-1000120 |
| Dual Index Kit TT Set A, 96 rxns | 10XGenomics | PN-1000215 |
| CircLigase™ ssDNA Ligase | Lucigen | CL4111K |
| T4 RNA Ligase 2, truncated | New England Biolabs | M0242L |
| StemSpan™ SFEM II | StemCell Technologies | 09655 |
| Fetal Bovine Serum - Premium Select G21162 | Bio-Techne | S11550 |
| SUPERase•In™ RNase Inhibitor (20 U/μL) | Invitrogen | AM2694 |
| EasySep Human Cord Blood CD34 Positive Selection Kit II | StemCell Technologies | 17896 |
| Immobilon-P PVDF Membrane | Millipore Sigma | IPVH00010 |
| SPRIselect | Beckman Coulter | B23317 |
| Qubit™ 1X dsDNA HS Assay Kit | ThermoFisher | Q33231 |
| Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells 25rxn | Invitrogen | 450031 |