Table 4.
A summary of representative studies evaluating the performance of real-time PCR and other methods.
| No | Country | Study population | Sensitivity |
References | |
|---|---|---|---|---|---|
| qPCR | Other methods | ||||
| 1 | Ethiopia | 844 school children | Sensitivity: S. stercoralis 73.9% | Formol-Ether concentration Technique (FECT): S. stercoralis 5.2% | (Hailu et al., 2022) |
| Spontaneous tube sedimentation technique (STST): S. stercoralis 10.3% | |||||
| Baermann concentration technique (BCT): S.stercoralis 26.4% | |||||
|
|
|
|
|
Agar plate culture (APC) : S. stercoralis 28.0% |
|
| 2 |
Fiji |
40 individuals |
Sensitivity qPCR compare to Kato- Katz: A. lumbricoides 75% and hookworms 66.7% |
|
(Azzopardi et al., 2021) |
| 3 |
Tanzania |
1636 individuals |
Sensitivity: T. trichiura 94.4%, A. lumbricoides 85.1%, and hookworm 85.2% |
Kato-Katz: T. trichiura 78.2%, A. lumbricoides 38.5% and hookworm 39.7% |
(Keller et al., 2020) |
| 4 |
Bangladesh |
2,799 children |
Sensitivity: A. lumbricoides 79%, hookworm 93%, and T. trichiura 90% |
Double-slide Kato-Katz: A. lumbricoides 49%, hookworm 32% and T. trichiura 52% |
(Benjamin-chung et al., 2020) |
| 5 |
Tanzania |
320 individuals |
Sensitivity: T. trichiura 89.1%, hookworm 72.7% and A. lumbricoides 87.5% |
Kato-Katz: T. trichiura 83.6%, hookworm 43.0% and A. lumbricoides 53.8% |
(Barda et al., 2020) |
| 6 | Brazil, Ethiopia, Lao PDR, and Tanzania | 645 children | Sensitivity: A. lumbricoides 90.0%, T. trichiura 94.7% and hookworm 91.9% | Single Kato-Katz: A. lumbricoides 71.9%, T. trichiura 88.1% and hookworm 72.6% Duplicate Kato-Katz: A. lumbricoides 73.0%, T. trichiura 90.9% and hookworm 75.3% | (Cools et al., 2019) |
| Mini-FLOTAC: A. lumbricoides 63.3%, T. trichiura 91.5% and hookworm 73.9% | |||||
|
|
|
|
|
FECPAKG2
A. lumbricoides 58.9%, T. trichiura 59.8% and hookworm 52.4% |
|
| 7 |
Timor Leste |
571 children (860 sample) |
Sensitivity: A. lumbricoides 94.1%, hookworm 75.7%, and T. trichiura 53.1% |
Sodium nitrate flotation (SNF): A. lumbricoides 68.1%, hookworm 66.9% and T. trichiura 81.3% |
(Clarke et al., 2018) |
| 8 |
Philippines |
263 school children |
Sensitivity: A. lumbricoides 60.8%, and T. trichiura 38.8% |
Kato-Katz: A. lumbricoides 20.5%, and T. trichiura 23.6% |
(Mationg et al., 2017) |
| 9 |
Kenya |
796 individuals |
Sensitivity: A. lumbricoides 98% and N. americanus 98% |
Kato-Katz: A. lumbricoides 70% and N. americanus 32% |
(Easton et al., 2016) |
| 10 | Côte d’Ivoire | 256 individuals | Sensitivity: S. stercoralis 76.8% | Koga agar plate: S. stercoralis 21.4% | (Becker et al., 2015) |
| Baermann: S. stercoralis 37.5% | |||||