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. 2022 Aug 10;13(2):577–597. doi: 10.1016/j.apsb.2022.07.019

Figure 8.

Figure 8

Honokiol improved the motor function, extended the lifespan, and alleviated the pathology of the SOD1-G93A transgenic mice. (A) The experimental process of in vivo studies; (B) The survival determination (n = 6–8); (C) The rotarod latency determination (n = 6–8); (D) The hanging wire test latency determination (n = 6–8); (E) Immunohistochemistry of ChAT positive MNs of the spinal cord (n = 3). Scale bars, 200 μm; (F) Western blot analysis and quantification of GFAP and IBA1 in the spinal cord tissue lysates (n = 4); (G) and (H) Representative images of GFAP and IBA1 immunofluorescence staining of the spinal cord. Scale bars, 100 μm; (I) Representative images of H&E staining of the gastrocnemius muscle. Scale bars, 100 μm. Kaplan–Meier analysis with log rank statistic test was used for the comparisons of the survival curves among the indicated groups (SPSS 16.0). Repeated measures ANOVA followed by post hoc LSD test was used for the comparisons of rotarod latency and hanging wire test latency determination among the indicated groups (SPSS 16.0). Student's t-test was used for the comparison of the ChAT positive MNs between SOD1-G93A mice and honokiol-treated mice (SPSS 16.0). One-way ANOVA followed by post hoc LSD test was used for the comparisons among the indicated groups in the Western blot quantification (SPSS 16.0). Data are presented as the mean ± SEM. ##P < 0.01, ###P < 0.001 versus WT control group; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 versus SOD1-G93A model group.