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Device validation and pressure limits. A. Pressure within each well of a 96-well plate. Each column was set to a static pressure ranging from 2.5 to 50 psi. B. Burst pressure of 3D-printed insert device. C. 3D CAD model of X-profile O-ring. D. Photograph of 3D-printed insert device showing PTFE backup rings above and below X-profile O-rings. E. Photograph of device within well of 96-well plate. F. 3D CAD model of cross-section of X-profile O-ring.
