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. Author manuscript; available in PMC: 2023 Mar 2.
Published in final edited form as: ACS Chem Biol. 2021 Sep 14;16(11):2315–2325. doi: 10.1021/acschembio.1c00475

Figure 2.

Figure 2.

Oligomeric and enzymatic characterization of individual PDX homo- and heterocomplexes. (a) Representative native MS spectrum for heterocomplex 3:1, zoomed into the 12mer region. Each symbol above the peak indicates one 12mer species, with their assignment, mass, and relative intensity shown on the right. Each peak with the same symbol is the same species carrying a different number of charges. Annotation was performed using UniDec.36 (b) Deconvoluted mass distributions of PDX 12mer complexes from different samples as labeled on the right. Color bars are overlaid to show the numbers of PDX1.2 and PDX1.3 protomers in the PDX 12mers as noted by the numbers on the top. (c) Histograms showing the enzymatic activities based on total PDX protein concentration. Error bars represent the standard deviations from triplicate experiments. The sample names match to the mass distributions shown horizontally in panel b. (d) Histograms showing the enzymatic activities normalized to the amount of PDX1.3 in each sample.