FIG. 2.

ETO and ETO-2 associate with corepressors. Cos-7 cells were transfected with HA-ETO or Myc–ETO-2, and cell lysates were immunoprecipitated (IP) with anti-mSin3A antibody (A) or were transfected with FLAG-tagged N-CoR and HA–ETO-2 or HA–ETO-2 alone and immunoprecipitated with anti-FLAG antibody (B). Proteins were detected by immunoblot analysis using anti-mSin3A, anti-HA, and anti-Myc antibodies. The bottom panels show the level of expression of ETO and ETO-2. The arrow indicates the presence of a background band.