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Mapping of the HDAC-binding sites on ETO. FLAG-tagged HDAC-1 to -3 were coexpressed with the GAL4-ETO chimeric proteins from Fig. 4. Copurifying GAL4-ETO proteins were detected using anti-GAL4 immunoglobulin G for immunoblot analysis. Shown are the control anti-FLAG antibody blot showing that each HDAC was expressed and immunoprecipitated (top), copurifying ETO fragments (middle), and the levels of expression of the GAL4-ETO chimeric proteins (bottom).
