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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: FASEB J. 2023 Mar;37(3):e22787. doi: 10.1096/fj.202201066R

Figure 6: SK1 and BMP4 are required for stabilization of Snail protein.

Figure 6:

A. Vector and SK1KO CRISPR cells were treated with 0.8 μM Dox or DMSO for 24 hours, and message level of SNAIL was measured by qRT-PCR (n=4,***=p<0.001). B. SNAIL qRT-PCR of MDA-MB-231 cells pre-treated with DMSO or 100 nM PF543 for 2hrs, and then treated with DMSO of 0.8 μM Dox for 24 hours (n=4,***= p<0.001). C. CRISPR SK1KO cells were pre-treated with 10 nM bortezomib (BTZ) to inhibit proteosome activity for 2 hours prior to 24-hour treatment with either DMSO or 0.8 μM Dox. Western blot was used to determine protein expression (n=3). D. Snail Western blot and qRT-PCR from MDA-MB-231 cells pretreated with 200 ng/ml of BMP4 inhibitor Noggin (nog) for 2 hours prior to 24-hour treatment with 0.8 μM Dox or DMSO (n=3). E. qRT-PCR of SK1 in MDA-MB-231 cells treated with 100 ng/ml Noggin for 2 hours prior to 24-hour treatment with 0.8 μM Dox (n=4).