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Time course of PARP cleavage following LA treatment. MCF10A cells grown in either complete or serum-free medium were treated with vehicle (0.1% ethanol) or LA (1 μM) for the indicated times. Cell lysates were subjected to SDS-PAGE and Western blotting using a polyclonal anti-PARP antibody which recognizes both full-length and cleaved PARP as indicated. The last two samples were treated with zVAD-fmk (25 μM) 30 min prior to the addition of LA to inhibit general caspase activation.
