FIG. 8.

Bcl-2 overexpression prevents LA-induced PARP cleavage in MCF10A cells. (A) Protein levels of Bcl-2 in MCF10A cells or three independent stable lines expressing either empty vector (pcDNA3, lanes a to c) or pcDNA3-Bcl2 (pcDNA3Bcl2, lanes a to c) were measured by Western blotting with a monoclonal antibody against Bcl-2. The blot was stripped and reprobed with a monoclonal antibody against β-actin to confirm equivalent loading. (B) Parental MCF10A cells or stable lines (described in panel A) were subjected to 1 μM LA treatment for 16 h in serum-free medium. PARP cleavage was assessed by Western blotting as in the experiment in Fig. 6.