FIG. 9.

Bcl-2 rescues detachment and loss of viability following LA treatment. (A) MCF10A cell lines stably expressing either vector (pcDNA3) or Bcl-2 (pcDNA3-Bcl2) were treated with either vehicle (0.1% ethanol) or 5 μM LA. Representative fields of each were photographed after 48 h. Bar, 10 μm. (B) MCF10A parental cell lines or those stably transfected with either pcDNA3 or pcDNA3-Bcl2 were grown in six-well dishes and treated with LA or left untreated, as in panel A, for 48 h. Loss of cell viability was measured by staining for trypan blue exclusion. Bars represent the mean and standard deviations of three independent experiments in which at least 200 cells were scored for cell death.