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. Author manuscript; available in PMC: 2023 Mar 2.
Published in final edited form as: Oncogene. 2022 Jun 27;41(30):3747–3760. doi: 10.1038/s41388-022-02382-y

Fig. 6: AR-DNA interaction decreases following NRP2 depletion:

Fig. 6:

A. RT-PCR of NKX3–1 and KLK3 was carried out under steady state and DHT induced condition following the depletion of NRP2. B-D. AR ChIP was carried out following the pulldown of crosslinking DNA with AR specific antibodies under steady state and DHT induced condition under the presence and absence of NRP2. Using pulldown DNA, RT-PCR was carried out with the primer of promoter sequences of these genes. Initially, NRP2 dependence on two AR-binding sites was assayed. In B, proximal AR-binding site (Site 1) has more effect on NRP2 depletion. KLK2 binding was also analyzed using the qPCR in C4–2B as well as C4–2 as Fig C and D. E-G: KLK3 promoter luciferase assay was carried out following the depletion of NRP2 or Nup93 (in F and G). Firefly luciferase activity is represented as relative luciferase unit (RLU).