. 2023 Feb 23;79(Pt 3):61–69. doi: 10.1107/S2053230X23001206

Table 2. Crystallization.

	Ac-FabH + OCoA	FabH + OCoA	CATIII + AcOCoA
Method	Vapor diffusion, hanging drop	Vapor diffusion, hanging drop	Vapor diffusion, hanging drop
Plate type	VDX	VDX	VDX
Temperature (K)	298	298	298
Protein concentration (µM)	680	680	960
Buffer composition of protein solution	10 mM AcOCoA, 200 mM NaCl, 10 mM Tris–HCl pH 8.0	10 mM AcOCoA, 200 mM NaCl, 10 mM Tris–HCl pH 8.0	10 mM AcOCoA, saturated chloramphenicol, 200 mM NaCl, 10 mM Tris–HCl pH 8.0
Composition of reservoir solution	75 mM MgCl₂, 20% PEG 6000, 3% PEG 400	1.5% DMSO, 75 mM MgCl₂, 23% PEG 3350, 100 mM HEPES–NaOH pH 7.5	100 mM MgCl₂, 46% PEG 400, 100 mM sodium citrate pH 5.5
Volume and ratio of drop	4 µl; 2:2 protein:well solution	4 µl; 2:2 protein:well solution	4 µl; 1:3 protein:well solution
Volume of reservoir (ml)	1.0	1.0	1.0