| Method |
Hanging-drop vapor diffusion |
Hanging-drop vapor diffusion |
| Plate type |
24-well VDX plate |
24-well VDX plate |
| Temperature (K) |
293 |
293 |
| Protein concentration (mg ml)−1
|
2.0–3.5 |
2.0–3.0 |
| Buffer composition of protein solution |
20 mM Tris–HCl pH 8.0, 150 mM NaCl, 5%(v/v) glycerol |
20 mM Tris–HCl pH 8.0, 150 mM NaCl, 5%(v/v) glycerol |
| Composition of reservoir solution |
A: 0.1 M HEPES–NaOH pH 7.73, 2.0–2.7 M ammonium formate |
B: 0.1 M HEPES–NaOH pH 7.73, 1.5–2.0 M magnesium sulfate heptahydrate |
C: 0.1 M Tris–HCl pH 7.5, 1.5–2.0 M magnesium sulfate heptahydrate |
0.1 M MES–NaOH pH 6.5–6.8, 25–30%(w/v) polyethylene glycol monomethyl ether 5000, 0.2 M ammonium sulfate |
| Volume and ratio of drop |
2 µl, 1:1 |
2 µl, 1:1 |
2 µl, 1:1 |
2 µl, 1:1 |
| Volume of reservoir (µl) |
500 |
500 |
500 |
500 |
