Compared to the PG/VG group, the nicotine group travelled significantly faster in both female (A;
p< 0.0001) and male (B;
p< 0.0001) mice. When separated into 1 min time bins, the nicotine group traveled significantly more distance in both females (C; p<0.0001) and males (D; p<0.0001). In females (E), there was no effect of nicotine on time spent in the center. However, in males (F), the nicotine group spent significantly more time in the center of the arena (p<0.001).
Data are expressed as mean ± SEM. Panels A-B and E-F utilized an unpaired a t test, whereas panels C-D were analyzed with a 2-way ANOVA. N=5-6 per group. *p< 0.05, *** p< 0.001, **** p< 0.0001