Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

[Preprint]. 2023 Feb 24:2023.02.23.529824. [Version 1] doi: 10.1101/2023.02.23.529824

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This work is licensed under a Creative Commons Attribution 4.0 International License, which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.

PMC Copyright notice

Fig. 1. — (A) Relative expression levels of Tex10 in mouse tissue/cell line samples (n=30). The male and female PGCs are indicated in orange and green, respectively. Data were obtained from the publicly available dataset (Hutchins et al. 2017).

(B) Relative expression levels of Tex10 on day 1, day 3, day 7 testes, and spermatocytes from adult testes. The data were curated from GSE83264.

(C) Schematic representation of the Tex10 degron system. Briefly, the Tex10 C terminus was edited using the CRISPR/Cas9 scissor to integrate degron system elements, and the Cre-loxP system was further used to delete the dsRed element.

(D) Western blot validation on two cell clones (C1 and C2) of the Tex10 degron system. The top arrow indicates Tex10-HA-tagged FKBP12^F36V protein with a size of around 118 kDa, and the second arrow indicates the wildtype Tex10 protein of 106 kDa.

(E) Cellular morphology of embryoid bodies (EBs) treated with DMSO or dTAG13 during six days of in vitro PGCLC specification from EpiLCs.

(F) Flow cytometry analysis of PGCLC specification efficiency using cell surface markers SSEA1 and CD61. Percentages of double-positive (SSEA1⁺ and CD61⁺) cells are indicated at day 2 and day 6 of PGCLC induction for clone C1.

(G-H) Quantification of double-positive (SSEA1⁺ and CD61⁺) percentage in live cells (G) and cell numbers per 20,000 analyzed cells (H) shown with bar plots. Two cell clones C1 and C2 were used as biological replicates, and an ANOVA test was used to detect significance.

(I-K) Flow cytometry analysis of PGCLC specification efficiency using cell surface markers SSEA1 and CD61. Percentages of double-positive (SSEA1⁺ and CD61⁺) cells are indicated at day 6 of PGCLC induction (I). Quantification of double-positive percentage in live cells (J) and cell numbers per 20,000 analyzed cells (K) are shown with bar plots. Two cell clones C1 and C2 were used as biological replicates, and a paired t-test was used to detect significance. Withdrawal for depleting Tex10 only at day one of PGCLC induction and then transferring EBs into medium without dTAG13 to restore Tex10 expression; No Withdrawal for depleting Tex10 for six days of PGCLC induction.