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[Preprint]. 2023 Feb 24:2023.02.23.529824. [Version 1] doi: 10.1101/2023.02.23.529824

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Fig. 5. — (A) Volcano plots depicting global transcriptomic changes induced by Tex10 OE at EpiLC, D2, D4, and D6 PGCLC stages.

(B) Bar plots showing expression changes of representative PGC regulators induced by Tex10 OE during the transition from EpiLC to D6PGCLC stage. The p-value was computed by edgeR using a generalized linear model-based statistical method.

(C) Gene set enrichment analysis of the effects of Tex10 OE on pluripotency, mesoderm, and ectoderm development at D2 and D4 PGCLC stages.

(D) Regression models exploring the correlation between NESs (normalized enrichment scores) of the positive regulation of Wnt signaling and PGC development, pluripotency regulation, mesoderm, and endoderm development.

(E) Flow cytometry analysis of the effect of the Wnt activator (CHIR: CHIR99021) on PGCLC specification efficiency in Tex10 OE cells. Percentages of double-positive (SSEA1+ and CD61+) cells are indicated at day 6 of PGCLC induction. Quantification of double-positive percentages in live cells and numbers per 20,000 analyzed cells are shown with bar plots. Two cell clones, OE1 and OE2, were used as biological replicates and the paired t-test was used to detect significance.