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[Preprint]. 2023 Feb 24:2023.02.23.529824. [Version 1] doi: 10.1101/2023.02.23.529824

Fig. 6. Tex10 cKO compromises male fertility.

Fig. 6.

(A) Immunofluorescence images showing protein expression of β-Galactosidase (the coding product of the LacZ gene) and Sox9 in wildtype and Tex10 LacZ/+ mouse testes. Hoechst 33342 shows the nucleus (scale bar, 50 μm).

(B) Schematic depiction of the mating strategy for generating cKO (Tex10 f/f;Stra8-iCre/+) and WT-equivalent (Tex10 f/f) male mice.

(C) Morphology and quantification of sperm count between cKO and WT male mice from the matings shown in B (scale bar, 25 μm).

(D) Comparison of litter sizes produced by Tex10 +/+ female mice mated with male WT and cKO mice from the matings shown in B.

(E-G) GSEA plots of spermatogenesis (E), Wnt/b-catenin signaling (F), and apoptosis (G) obtained using RNA-seq data from testes of WT and Tex10 cKO mice from the matings shown in B.

(H) Bar plots showing gene expression changes related to spermatogenesis, sperm motility, apoptosis, and Wnt signaling between WT and Tex10 cKO mice from matings shown in B.